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A Novel Recombinant Lys-C Protease for Proteomic Sample Preparation

Part # PS129

Abstract

A Novel Recombinant Lys-C Protease for Proteomic Sample Preparation
Sergei V. Saveliev1, James R. Hartnett1, Rebecca L. Godat1, Alexander S. Hubert2, Joshua J. Coon2 and Marjeta Urh1
1Promega Corporation, Madison, WI; 2UW-Madison, Madison, WI

Owing to efficient proteolysis and particular advantages of trypsin generated peptides for mass spectrometry analysis, trypsin is the most widely used proteomic protease. Recently, however, Lys-C has been increasingly used as either a trypsin alternate or as supplement. Its increasing favor is largely due to its ability to perform proteolytic digestion under protein denaturing conditions, an attribute that can greatly extend the observable proteome.
Lys-C is found in a number of bacterial hosts with Lysobacter enzymogenes being used as a most popular source of commercially available Lys-C. We have now developed a recombinant form of Lys-C from Pseudomonas aeruginosa. In this study we compare performance of Pseudomonas and Lysobacter Lys-C and demonstrate benefits of Pseudomonas Lys-C for proteomic analysis.

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