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A Homogeneous Bioluminescent System for Measuring GTPase, GAP and GEF Activities

Part # PS265

Abstract

Subhanjan Mondal, Kevin Hsiao, Said A. Goueli
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI, 53711

Targeting GTPases and their regulators has been challenging due to lack of convenient assays. To overcome the challenges in analyzing activities of GTPases and their regulatory proteins, GTPase Activating Proteins (GAP) and Guanine Nucleotide Exchange Factors (GEF), we have developed a homogeneous bioluminescent assay (GTPase/GAP/GEF-Glo) system to analyze these proteins in a simple, convenient “add-mix-read” format.

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