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A Flexible Workflow for Automated Bioluminescent Kinase Selectivity Profiling Poster

Part # PS244

Abstract

Matt Butzler1, Tracy Worzella1, Jacquelyn Hennek1, Seth Hanson2, Karen Kleman2, Said Goueli1, Hicham Zegzouti1, Mark Bratz1 and Cristopher Cowan1
1Promega Corporation, 2800 Woods Hollow Rd, Madison WI, 53711, USA; 2Gilson Inc., Middleton WI, USA

Kinase profiling is a necessary process to confirm inhibitor selectivity and assess off-target activity. The arduous task of optimizing kinase reactions and maintaining a library of enzymes often results in the outsourcing of kinase profiling activities. We aim to show how ready-to-use Kinase Selectivity Profiling Systems (KSPS) combined with easy-to-use instrumentation and automated data analysis supports a streamlined workflow to enable quick and efficient in-house kinase profiling.

Printed in USA.