A Bioluminescent Cell Viability Assay Optimized for 3D Microtissues

Part # PS210

Abstract

Michael P. Valley, Chad Zimprich, James J. Cali, and Dan F. Lazar
Promega Corporation, 2800 Woods Hollow Road, Madison WI, 53711-5399, USA
AACR 2013 Abstract #5531
Cells assessed in 3D culture models frequently provide more physiologically relevant data than cells studied in standard 2D formats. Thus, there is a need for convenient and effective assays explicitly validated for 3D microtissues. Here we report on CellTiter-Glo® 3D, a bioluminescent ATP detection assay for measuring cell viability with an optimized protocol and an improved formulation that has been expressly designed to measure the viability of 3D microtissues. This single-component liquid reagent has significant lytic capacity, exhibits high ATP recovery, and can be used to measure the viability of microtissues grown in a variety of 3D culture models, including ECM-independent (e.g., hanging drop), ECM-dependent (e.g., Matrigel™), and synthetic scaffolds (e.g., Alvetex™).

Download the poster, below. See also the CellTiter-Glo® 3D product page.

Printed in USA.