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A Novel 6 x 5 Peptide Mix for LC-MS/MS Performance Monitoring

Part # PS221


Michael Rosenblatt, Ethan Strauss, and Marjeta Urh
Promega Corporation, 2800 Woods Hollow Road, Madison WI 53711

Performance monitoring of LC-MS/MS instrumentation continues to be challenging and has yet to be standardized across all MS laboratories. Here we report the development of a novel reference peptide mixture. In combination with an accompanying software tool, the mixture reports on LC column performance and MS instrument parameters, including sensitivity and dynamic range. In addition, the software can also report on parameter history, compare instruments, and display XIC traces for the peptides. This is the first example of a peptide mixture designed to give a report of all critical LC and MS parameters in a single run.

Printed in USA.