Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

[U-13C, 15N] Protein Labeling Using Escherichia coli Strain KRX

Kate Qin Zhao, Marco Tonelli, Jim Hartnett, and Michael Slater
1Promega Corporation, 2NMRFAM, University of Wisconsin-Madison
Publication Date: 2008


We have developed a protocol for efficient [U-13C, 15N]labeled protein production in the highly competent E. coli strain, KRX, using Silantes CN OD2 medium. This procedure can facilitate protein analysis by NMR spectroscopy and other methodologies that require 13C and 15N protein labeling.

Promega Notes 98, 6–7.