Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Success

Thank you for verifying your email address.

Email Verification Error

There was an issue verifying your email address. Please try again or contact Customer Service.

Contact Customer Service

United States

TSAP: A New Thermosensitive Alkaline Phosphatase

John Brandon and Michael R. Slater
Promega Corporation
Publication Date: 2007

Abstract

Here we describe TSAP, a new thermosensitive alkaline phosphatase. TSAP is active in all Promega restriction enzyme buffers, including MULTI-CORE™ Buffer, as well as both GoTaq® Buffers. The robust phosphatase activity effectively dephosphorylates all DNA termini (5´, 3´ and blunt) in 15 minutes at 37°C. As TSAP is active in restriction enzyme buffers, using TSAP offers the potential for simultaneous restriction digestion/dephosphorylation. Promega TSAP is thoroughly tested to ensure functionality and physical purity.

Promega Notes 95, 3–4.

Abstract Article Related Products Related Protocols Related Resources Related Articles
Choose your country

Americas

Brazil
/
Canada
/
United States

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
Estonia
Finland
France
/
Germany
/
Iceland
Italy
/
Luxembourg
Netherlands
Norway
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom
Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.