Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

TSAP: A New Thermosensitive Alkaline Phosphatase

John Brandon and Michael R. Slater
Promega Corporation
Publication Date: 2007


Here we describe TSAP, a new thermosensitive alkaline phosphatase. TSAP is active in all Promega restriction enzyme buffers, including MULTI-CORE™ Buffer, as well as both GoTaq® Buffers. The robust phosphatase activity effectively dephosphorylates all DNA termini (5´, 3´ and blunt) in 15 minutes at 37°C. As TSAP is active in restriction enzyme buffers, using TSAP offers the potential for simultaneous restriction digestion/dephosphorylation. Promega TSAP is thoroughly tested to ensure functionality and physical purity.

Promega Notes 95, 3–4.