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Identify P-glycoprotein Substrates and Inhibitors with the Rapid HTS Pgp-Glo™ Assay System

Dongping Ma and James J. Cali
Promega Corporation
Publication Date: 2007

Abstract

The Pgp-Glo™ Assay Systems are luminescent P-glycoprotein (Pgp) ATPase assays. The Pgp-Glo™ Assay detects the effects of compounds on recombinant human Pgp in a cell membrane fraction. The assay couples the ATPase activity of Pgp to the ATP dependence of the light-generating reaction of firefly luciferase. The rate of ATP consumption by Pgp is inversely correlated with the light output of the luciferase reaction. The Pgp-Glo™ Assay is easily amenable to high-throughput screening and can complement cell-based Pgp studies.

Promega Notes 96, 11–14.

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