We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

A Novel System for Regulated Protein Expression in Mammalian Cells

  • Print
  • Email

Abstract

We describe the Regulated Mammalian Expression System, which allows researchers to achieve tightly controlled protein expression in mammalian cells. This system uses coumerin-based compounds to modulate regulation of gene expression while maintaining high levels of induction over low levels of basal activity. In addition, expression of the gene of interest can be turned off rapidly by novobiocin addition. Introduction of a unique auto-amplification loop for transactivator expression makes the system robust and minimizes potential undesired effects on host gene expression.

Promega Notes 95, 8–11.

Jolanta Vidugiriene, Jim Hartnett, Elaine Schenborn, and Michael R. Slater

Promega Corporation
Publication Date: 2007

Download Article (229 KB)