PureYield™ RNA Midiprep System: Isolating Pure Total RNA without DNase

Paula Brisco, Michelle Mandrekar, Rex Bitner, Katharine Hoffmann and Nadine Nassif
Promega Corporation
Publication Date: 2006

Abstract

The PureYield™ RNA Midiprep System is designed to isolate high-quality total RNA with high yields from essentially any type of sample. Efficient DNA removal is integrated into a streamlined, universal protocol and is accomplished without DNase treatment or phenol:chloroform extractions. This novel approach results in pure total RNA that is essentially free of genomic DNA. The purified total RNA is ready for use in downstream applications, such as qRT-PCR and microarray analysis.

Promega Notes 92, 14–19.