HaloLink™ Resin provides a new method for specific, covalent and oriented immobilization of proteins onto surfaces. The strategy is based on the HaloTag® Protein, which is derived from a catalytically inactive hydrolase engineered to form a covalent bond with a specific ligand. Here we demonstrate the high binding capacity and minimal nonspecific binding of the HaloLink™ Resin. We also show that the covalent HaloTag® bond provides stability during dilution and stringent washing, minimizing the loss of the HaloTag® fusion proteins from the surface. By analyzing protein:protein interactions and enzyme activity for several fusion proteins, we also demonstrate that fusion proteins bound to the HaloLink™ Resin maintain functionality. HaloLink™ Resin is not a purification resin; however, in conjunction with protease cleavage, it can be used to isolate a protein of interest.
Promega Notes 92, 24–29.