Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

A New System for Cloning and Expressing Protein-Coding Regions

Michael Slater, Jim Hartnett, Natalie Betz, Jami English, Ethan Strauss, Becky Pferdehirt and Elaine Schenborn
Promega Corporation
Publication Date: 2005


The Flexi® Vector Systems provide a rapid, efficient and high-fidelity method to transfer protein-coding regions between vectors with various expression or peptide tag options. Inserts are efficiently transferred between Flexi® Vectors following restriction enzyme digestion, maintaining insert orientation and reading frame. During the initial cloning step, a lethal gene in the Flexi® Vector cloning region allows positive selection for ligation of the insert. Antibiotic resistance genes simplify selection of the desired clone during subsequent transfer to other Flexi® Vectors.

Promega Notes 89, 11–15.

Related Products

Related Resources