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RNA Purification: A Rapid and Versatile Protocol for the Isolation of Total RNA

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Abstract

Promega's new SV Total RNA Isolation System offers a complete system for the isolation of quality RNA from small amounts of starting material. This system provides a fast and simple technique for the preparation of purified and intact total RNA from tissues, cultured cells and whole blood samples. This purification is achieved without the use of phenol/chloroform extractions and employs a simple DNase step to eliminate genomic DNA efficiently. No genomic DNA contamination is seen when the RNA is analyzed by reverse transcription-PCR (RT-PCR). In this article, we demonstrate that RNA isolated using this system is highly pure and is ideally suitable for applications such as RT-PCR.

Promega Notes 64, 7.

Paula Brisco, Jacqui Sankbeil and Dan Kephart

Promega Corporation
Publication Date: 1997

Download Article (253 KB)

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