This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pNLCoI4[luc2-P2A-NlucP/PGK/Hygro] Vector sequence reference points: Base pairs 7025 PGK promoter 27–541 luc2 reporter gene 577–2226 P2A region 2227–2292 NlucP reporter gene 2293–2931 SV40 late poly(A) region 2971–3192 SV40 early enhancer/promoter 3240–3658 Hygromycin resistance coding region 3683–4720 Synthetic poly(A) sequence 4744–4792 Reporter Vector primer 4 (RVprimer4) binding region 4859–4878 Col EI-derived plasmid replication origin 5116 Synthetic β-lactamase (Ampr) coding region 5907–6767 Synthetic poly(A) signal/transcriptional pause region 6872–7025 Reporter Vector primer 3 (RVprimer3) binding region 6974–6993 1 GGCCTAACTG GCCGGTACCT GAGTCTTCCA CGGGGTTGGG GTTGCGCCTT 51 TTCCAAGGCA GCCCTGGGTT TGCGCAGGGA CGCGGCTGCT CTGGGCGTGG 101 TTCCGGGAAA CGCAGCGGCG CCGACCCTGG GTCTCGCACA TTCTTCACGT 151 CCGTTCGCAG CGTCACCCGG ATCTTCGCCG CTACCCTTGT GGGCCCCCCG 201 GCGACGCTTC CTCGTCCGCC CCTAAGTCGG GAAGGTTCCT TGCGGTTCGC 251 GGCGTGCCGG ACGTGACAAA CGGAAGCCGC ACGTCTCACT AGTACCCTCG 301 CAGACGGACA GCGCCAGGGA GCAATGGCAG CGCGCCGACC GCGATGGGCT 351 GTGGCCAATA GCGGCTGCTC AGCAGGGCGC GCCGAGAGCA GCGGCCGGGA 401 AGGGGCGGTG CGGGAGGCGG GGTGTGGGGC GGTAGTGTGG GCCCTGTTCC 451 TGCCCGCGCG GTGTTCCGCA TTCTGCAAGC CTCCGGAGCG CACGTCGGCA 501 GTCGGCTCCC TCGTTGACCG AATCACCGAC CTCTCTCCCC AAAGCTTGGC 551 AATCCGGTAC TGTTGGTAAA GCCACCATGG AAGATGCCAA AAACATTAAG 601 AAGGGCCCAG CGCCATTCTA CCCACTCGAA GACGGGACCG CCGGCGAGCA 651 GCTGCACAAA GCCATGAAGC GCTACGCCCT GGTGCCCGGC ACCATCGCCT 701 TTACCGACGC ACATATCGAG GTGGACATTA CCTACGCCGA GTACTTCGAG 751 ATGAGCGTTC GGCTGGCAGA AGCTATGAAG CGCTATGGGC TGAATACAAA 801 CCATCGGATC GTGGTGTGCA GCGAGAATAG CTTGCAGTTC TTCATGCCCG 851 TGTTGGGTGC CCTGTTCATC GGTGTGGCTG TGGCCCCAGC TAACGACATC 901 TACAACGAGC GCGAGCTGCT GAACAGCATG GGCATCAGCC AGCCCACCGT 951 CGTATTCGTG AGCAAGAAAG GGCTGCAAAA GATCCTCAAC GTGCAAAAGA 1001 AGCTACCGAT CATACAAAAG ATCATCATCA TGGATAGCAA GACCGACTAC 1051 CAGGGCTTCC AAAGCATGTA CACCTTCGTG ACTTCCCATT TGCCACCCGG 1101 CTTCAACGAG TACGACTTCG TGCCCGAGAG CTTCGACCGG GACAAAACCA 1151 TCGCCCTGAT CATGAACAGT AGTGGCAGTA CCGGATTGCC CAAGGGCGTA 1201 GCCCTACCGC ACCGCACCGC TTGTGTCCGA TTCAGTCATG CCCGCGACCC 1251 CATCTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTC AGCGTGGTGC 1301 CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC 1351 GGCTTTCGGG TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG 1401 CAGCTTGCAA GACTATAAGA TTCAATCTGC CCTGCTGGTG CCCACACTAT 1451 TTAGCTTCTT CGCTAAGAGC ACTCTCATCG ACAAGTACGA CCTAAGCAAC 1501 TTGCACGAGA TCGCCAGCGG CGGGGCGCCG CTCAGCAAGG AGGTAGGTGA 1551 GGCCGTGGCC AAACGCTTCC ACCTACCAGG CATCCGCCAG GGCTACGGCC 1601 TGACAGAAAC AACCAGCGCC ATTCTGATCA CCCCCGAAGG GGACGACAAG 1651 CCTGGCGCAG TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTGGA 1701 CTTGGACACC GGTAAGACAC TGGGTGTGAA CCAGCGCGGC GAGCTGTGCG 1751 TCCGTGGCCC CATGATCATG AGCGGCTACG TTAACAACCC CGAGGCTACA 1801 AACGCTCTCA TCGACAAGGA CGGCTGGCTG CACAGCGGCG ACATCGCCTA 1851 CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG AAGAGCCTGA 1901 TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GAGCATCCTG 1951 CTGCAACACC CCAACATCTT CGACGCCGGG GTCGCCGGCC TGCCCGACGA 2001 CGATGCCGGC GAGCTGCCCG CCGCAGTCGT CGTGCTGGAA CACGGTAAAA 2051 CCATGACCGA GAAGGAGATC GTGGACTATG TGGCCAGCCA GGTTACAACC 2101 GCCAAGAAGC TGCGCGGTGG TGTTGTGTTC GTGGACGAGG TGCCTAAAGG 2151 ACTGACCGGC AAGTTGGACG CCCGCAAGAT CCGCGAGATT CTCATTAAGG 2201 CCAAGAAGGG CGGCAAGATC GCCGTGGGAA GCGGAGCTAC TAACTTCAGC 2251 CTGCTGAAGC AGGCTGGAGA CGTGGAGGAG AACCCTGGAC CTATGGTCTT 2301 CACACTCGAA GATTTCGTTG GGGACTGGCG ACAGACAGCC GGCTACAACC 2351 TGGACCAAGT CCTTGAACAG GGAGGTGTGT CCAGTTTGTT TCAGAATCTC 2401 GGGGTGTCCG TAACTCCGAT CCAAAGGATT GTCCTGAGCG GTGAAAATGG 2451 GCTGAAGATC GACATCCATG TCATCATCCC GTATGAAGGT CTGAGCGGCG 2501 ACCAAATGGG CCAGATCGAA AAAATTTTTA AGGTGGTGTA CCCTGTGGAT 2551 GATCATCACT TTAAGGTGAT CCTGCACTAT GGCACACTGG TAATCGACGG 2601 GGTTACGCCG AACATGATCG ACTATTTCGG ACGGCCGTAT GAAGGCATCG 2651 CCGTGTTCGA CGGCAAAAAG ATCACTGTAA CAGGGACCCT GTGGAACGGC 2701 AACAAAATTA TCGACGAGCG CCTGATCAAC CCCGACGGCT CCCTGCTGTT 2751 CCGAGTAACC ATCAACGGAG TGACCGGCTG GCGGCTGTGC GAACGCATTC 2801 TGGCGAATTC TCACGGCTTT CCGCCTGAGG TTGAAGAGCA AGCCGCCGGT 2851 ACATTGCCTA TGTCCTGCGC ACAAGAAAGC GGTATGGACC GGCACCCAGC 2901 CGCTTGTGCT TCAGCTCGCA TCAACGTCTA AGGCCGCGAC TCTAGAGTCG 2951 GGGCGGCCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG 3001 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT 3051 TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT 3101 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT 3151 GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCGAT 3201 AAGGATCCGT TTGCGTATTG GGCGCTCTTC CGCTGATCTG CGCAGCACCA 3251 TGGCCTGAAA TAACCTCTGA AAGAGGAACT TGGTTAGCTA CCTTCTGAGG 3301 CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC 3351 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 3401 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA 3451 AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC 3501 CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 3551 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA 3601 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 3651 AAAAGCTCGA TTCTTCTGAC ACTAGCGCCA CCATGAAGAA GCCCGAACTC 3701 ACCGCTACCA GCGTTGAAAA ATTTCTCATC GAGAAGTTCG ACAGTGTGAG 3751 CGACCTGATG CAGTTGTCGG AGGGCGAAGA GAGCCGAGCC TTCAGCTTCG 3801 ATGTCGGCGG ACGCGGCTAT GTACTGCGGG TGAATAGCTG CGCTGATGGC 3851 TTCTACAAAG ACCGCTACGT GTACCGCCAC TTCGCCAGCG CTGCACTACC 3901 CATCCCCGAA GTGTTGGACA TCGGCGAGTT CAGCGAGAGC CTGACATACT 3951 GCATCAGTAG ACGCGCCCAA GGCGTTACTC TCCAAGACCT CCCCGAAACA 4001 GAGCTGCCTG CTGTGTTACA GCCTGTCGCC GAAGCTATGG ATGCTATTGC 4051 CGCCGCCGAC CTCAGTCAAA CCAGCGGCTT CGGCCCATTC GGGCCCCAAG 4101 GCATCGGCCA GTACACAACC TGGCGGGATT TCATTTGCGC CATTGCTGAT 4151 CCCCATGTCT ACCACTGGCA GACCGTGATG GACGACACCG TGTCCGCCAG 4201 CGTAGCTCAA GCCCTGGACG AACTGATGCT GTGGGCCGAA GACTGTCCCG 4251 AGGTGCGCCA CCTCGTCCAT GCCGACTTCG GCAGCAACAA CGTCCTGACC 4301 GACAACGGCC GCATCACCGC CGTAATCGAC TGGTCCGAAG CTATGTTCGG 4351 GGACAGTCAG TACGAGGTGG CCAACATCTT CTTCTGGCGG CCCTGGCTGG 4401 CTTGCATGGA GCAGCAGACT CGCTACTTCG AGCGCCGGCA TCCCGAGCTG 4451 GCCGGCAGCC CTCGTCTGCG AGCCTACATG CTGCGCATCG GCCTGGATCA 4501 GCTCTACCAG AGCCTCGTGG ACGGCAACTT CGACGATGCT GCCTGGGCTC 4551 AAGGCCGCTG CGATGCCATC GTCCGCAGCG GGGCCGGCAC CGTCGGTCGC 4601 ACACAAATCG CTCGCCGGAG CGCAGCCGTA TGGACCGACG GCTGCGTCGA 4651 GGTGCTGGCC GACAGCGGCA ACCGCCGGCC CAGTACACGA CCGCGCGCTA 4701 AGGAGGTAGG TCGAGTTTAA ACTCTAGAAC CGGTCATGGC CGCAATAAAA 4751 TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGTTCGAACT 4801 AGATGCTGTC GACCGATGCC CTTGAGAGCC TTCAACCCAG TCAGCTCCTT 4851 CCGGTGGGCG CGGGGCATGA CTATCGTCGC CGCACTTATG ACTGTCTTCT 4901 TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCTCTT CCGCTTCCTC 4951 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 5001 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 5051 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 5101 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 5151 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 5201 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 5251 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 5301 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 5351 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 5401 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 5451 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 5501 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 5551 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 5601 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 5651 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 5701 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 5751 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC 5801 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 5851 TATATATGAG TAAACTTGGT CTGACAGCGG CCGCAAATGC TAAACCACTG 5901 CAGTGGTTAC CAGTGCTTGA TCAGTGAGGC ACCGATCTCA GCGATCTGCC 5951 TATTTCGTTC GTCCATAGTG GCCTGACTCC CCGTCGTGTA GATCACTACG 6001 ATTCGTGAGG GCTTACCATC AGGCCCCAGC GCAGCAATGA TGCCGCGAGA 6051 GCCGCGTTCA CCGGCCCCCG ATTTGTCAGC AATGAACCAG CCAGCAGGGA 6101 GGGCCGAGCG AAGAAGTGGT CCTGCTACTT TGTCCGCCTC CATCCAGTCT 6151 ATGAGCTGCT GTCGTGATGC TAGAGTAAGA AGTTCGCCAG TGAGTAGTTT 6201 CCGAAGAGTT GTGGCCATTG CTACTGGCAT CGTGGTATCA CGCTCGTCGT 6251 TCGGTATGGC TTCGTTCAAC TCTGGTTCCC AGCGGTCAAG CCGGGTCACA 6301 TGATCACCCA TATTATGAAG AAATGCAGTC AGCTCCTTAG GGCCTCCGAT 6351 CGTTGTCAGA AGTAAGTTGG CCGCGGTGTT GTCGCTCATG GTAATGGCAG 6401 CACTACACAA TTCTCTTACC GTCATGCCAT CCGTAAGATG CTTTTCCGTG 6451 ACCGGCGAGT ACTCAACCAA GTCGTTTTGT GAGTAGTGTA TACGGCGACC 6501 AAGCTGCTCT TGCCCGGCGT CTATACGGGA CAACACCGCG CCACATAGCA 6551 GTACTTTGAA AGTGCTCATC ATCGGGAATC GTTCTTCGGG GCGGAAAGAC 6601 TCAAGGATCT TGCCGCTATT GAGATCCAGT TCGATATAGC CCACTCTTGC 6651 ACCCAGTTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCGGGGTGTG 6701 CAAAAACAGG CAAGCAAAAT GCCGCAAAGA AGGGAATGAG TGCGACACGA 6751 AAATGTTGGA TGCTCATACT CGTCCTTTTT CAATATTATT GAAGCATTTA 6801 TCAGGGTTAC TAGTACGTCT CTCAAGGATA AGTAAGTAAT ATTAAGGTAC 6851 GGGAGGTATT GGACAGGCCG CAATAAAATA TCTTTATTTT CATTACATCT 6901 GTGTGTTGGT TTTTTGTGTG AATCGATAGT ACTAACATAC GCTCTCCATC 6951 AAAACAAAAC GAAACAAAAC AAACTAGCAA AATAGGCTGT CCCCAGTGCA 7001 AGTGCAGGTG CCAGAACATT TCTCT Sequence file created 20-November-2014.