We believe this site might serve you best:

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

What are some of the differences between Renilla luciferase and firefly luciferase?

SEARCH ARTICLES

Related Products

LabFact #6

For the Dual-Luciferase® Reporter Assay System, only use Passive Lysis Buffer since PLB is specially formulated to minimize background autoluminescence.
(from #TM040)

  • Print
  • Email

Firefly luciferase is a 61kDa protein isolated from beetles (Photinus pyralis), while Renilla luciferase is a 36kDa protein from sea pansy (Renilla reniformis).

These enzymes differ in their substrate and cofactor requirements. Firefly luciferase uses luciferin in the presence of oxygen, ATP and magnesium to produce light, while Renilla luciferase requires only coelenterazine and oxygen. Firefly luciferase produces a greenish yellow light in the 550–570nm range. Renilla luciferase produces a blue light of 480nm. These enzymes can be used in dual-reporter assays due to their differences in substrate requirements and light output.

More detailed information on luciferases can be found in the Bioluminescent Reporters chapter of the Promega Protocols & Applications Guide.

Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom