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Wizard® SV Gel and PCR Clean-Up System Technical Bulletin

Instructions for Use of Product(s)
A9281, A9282, A9285

Literature # TB308

The Wizard® SV Gel and PCR Clean-Up System extracts DNA fragments of 100bp to 10kb from standard or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE) and purifies PCR products directly from an amplification reaction. Up to 95% recovery is achieved, depending on DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, which can bind up to 40µg of DNA, allows recovery of isolated DNA fragments or PCR products in as little as 20 minutes, depending on the number of samples processed and protocol used. Purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.

Summary of Change
The following change was made to the 8/19 revision of this document:
Updated Section 5.A, Step 5.

Printed in USA. Revised 8/19.

Experienced User Protocols