Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

TnT® T7 Quick for PCR DNA

Coupled Transcription/Translation System for Optimum Protein Expression from PCR Templates

  • Express protein directly from PCR, no cleanup needed
  • Up to 5 times more protein than standard translation reactions with linear templates
  • Includes Recombinant RNasin® Ribonuclease Inhibitor

GloMore in Your Research with GloMax Plate Readers!

Get 20% off using GLOMORE2021 now through December 31st, 2021. Shop Now ›

Apply for the DOORS Scholarship Today!

Promega’s Diversification of Our Research Scientists (D.O.O.R.S) Scholarship recognizes and empowers underrepresented students studying life science by offering a $5,000 award and mentorship program. Applications are open through September 30, 2021.

Size

Catalog number selected: L5540

$ 682.00
Your price:
Please Enquire
This product is discontinued
TnT® T7 Quick for PCR DNA
40 reactions
$ 682.00
Your price: Log in

Protein from PCR Templates, No Clean Up Required

The TnT® T7 Quick for PCR DNA System is a simple, single-tube coupled transcription/translation system designed for optimum protein expression directly from PCR templates, no cleanup needed. For most PCR templates, the reactions produce up to 5 times more protein than other commercially available kits. The PCR-generated DNA can be used directly from the amplification reaction or purified by numerous commercially available kits and traditional methods.

Reagents including Recombinant RNasin® Ribonuclease Inhibitor are included in the Quick Master Mix.

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

Reference

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Methionine

L118A 1 × 50μl 1mM

TnT® T7 PCR Quick Master Mix

L554A 8 × 200μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

Choose language:

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Let's find the product that meets your needs.

Talk to a Scientist

Paraj

Paraj

US