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Luciferase Assay System

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Rapid Detection of Firefly Luciferase Activity

  • Linear over eight or more orders of magnitude of enzyme concentration
  • Sensitive to 10–20 moles of luciferase
  • Cell lysis, sample prep and assay complete in as little as 5 minutes

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Add Reporter Lysis Buffer

Catalog number selected: E1500

$ 125.00
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Luciferase Assay System
100 assays
$ 125.00
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Simple Non-Homogenous Assay to Quantify Firefly Luciferase Activity 

The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10–20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.

The Luciferase Assay Reagent generates light that is nearly constant for at least 1 minute and so the assay is compatible with measurement of luciferase using a single-tube luminometer or a multiwell plate luminometer with an auto-injector. A luminometer is preferred, but not required as the assay is adaptable to scintillation counters.

 

Cell Lysis Reagents used with the Luciferase Assay System

The Luciferase Assay System is a nonhomogeneous assay; the cells containing the luciferase must be lysed before reagent addition. Glo Lysis Buffer (Cat.# E2661), Cell Culture Lysis Reagent (Cat.# E1531), Passive Lysis Buffer (Cat.# E1941) and Reporter Lysis Buffer (Cat.# E3971) can be used with the Luciferase Assay System for reporter quantitation in mammalian cells. Reporter Lysis Buffer enables luciferase, CAT and β-galactosidase assays to be performed from the same cell extract. The Luciferase Assay System also can be used for quantitation in plant and bacterial cells, but only Cell Culture Lysis Reagent is suitable for these applications.

In some kits the lysis buffer is included, and in others it must be purchased separately (see the Specifications section of this page to view assay components).

References

  1. Wood, K.V. (1991) In: Bioluminescence & Chemiluminescence: Current Status, eds. P. Stanley and L. Kricka, John Wiley & Sons, Ltd., Chichester, 543.
  2. Seliger, H.H. and McElroy, W.D. (1960) Arch. Biochem. Biophys. 88, 136.
  3. Wood, K.V. et al. (1984) Biochem. Biophys. Res. Comm. 124, 592–6.
  4. de Wet, J.R. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7870–3.
  5. Alam, J. and Cook, J.L. (1990) Anal. Biochem. 188, 245–54.
  6. Schenborn, E. and Goiffon, V. (1993) Promega Notes 41, 11–3.
  7. Jones, D.P. et al. (1995) Promega Notes 54, 20–5.

Check out these newer, homogenous firefly luciferase assay options!!

ONE-Glo™ EX Luciferase Assay System

  • Moderate signal.
  • Moderate half-life (2 hours).
  • Ideal for high- or ultrahigh-throughput processing.

Steady-Glo® Luciferase Assay System

  • Reduced signal.
  • Maximum signal stability (5 hours).
  • Ideal for high-throughput applications when extended luminescence is required.

Bright-Glo® Luciferase Assay System

  • Brightest signal.
  • Short half-life (30 minutes).
  • Ideal for high-throughput applications when high sensitivity is required.

Specifications

You are viewing: E1500 Change Configuration

What's in the box?

Item Part # Size Available Separately

Luciferase Assay Substrate

E151A 1 × 1 vial

Luciferase Assay Buffer

E152A 1 × 10ml

Cell Culture Lysis 5X Reagent

E153A 1 × 30ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E4030 Change Configuration

What's in the box?

Item Part # Size Available Separately

Luciferase Assay Substrate

E151A 1 × 1 vial

Luciferase Assay Buffer

E152A 1 × 10ml

Reporter Lysis 5X Buffer

E397A 1 × 30ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E1501 Change Configuration

What's in the box?

Item Part # Size

Luciferase Assay Substrate

E151A 10 × 1 vial

Luciferase Assay Buffer

E152A 10 × 10ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E4530 Change Configuration

What's in the box?

Item Part # Size Available Separately

Luciferase Assay Substrate

E151A 10 × 1 vial

Luciferase Assay Buffer

E152A 10 × 10ml

Reporter Lysis 5X Buffer

E397A 10 × 30ml View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: E4550 Change Configuration

What's in the box?

Item Part # Size

Luciferase Assay Substrate

E151C 1 × 1 vial

Luciferase Assay Buffer

E152B 1 × 105ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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