Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

pGL4 Vectors: A New Generation of Luciferase Reporter Vectors

Aileen Paguio, Brian Almond, Frank Fan, Pete Stecha, Denise Garvin, Monika Wood and Keith Wood
Promega Corporation
Publication Date: 2005

Abstract

Reporter gene technologies are powerful tools by which to study gene expression and regulation. Among reporters used in mammalian cells, the luciferases are preferred because they are highly sensitive, flexible, easy to quantify and detectable over a broad dynamic range. We have developed several new reporter designs for firefly and Renilla luciferases, which allow a range of uses from measurement of transcriptional activity in cell biology applications to high-throughput screening in drug discovery. The resulting vectors yield higher expression with decreased signal-to-background ratios and respond more rapidly to transcriptional dynamics. The vectors are also designed to reduce the risk of anomalous effects.

Promega Notes 89, 7–10.