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Six Luminescent Substrates for Cytochrome P450 Assays

Promega Corporation
Publication Date: 2009

Abstract

Luminogenic CYP assays use prosubstrates for the light-generating reaction of firefly luciferase. CYPs convert the prosubstrates to an active luciferin, which produces light in a second reaction with luciferase. The amount of light generated is proportional to the amount of luciferin produced by the CYP and, therefore, to CYP enzyme activity.

Cell Notes 23, 19–20.

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