Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Clone and Express Protein-Coding Regions Using the Flexi® Vector Systems

Michael Slater, Jim Harnett, Natalie Betz, Jami English, Ethan Strauss, Becky Pferdehirt, and Elaine Schenborn
Promega Corporation
Publication Date: 2005

Abstract

The Flexi® Vector Systems provide a rapid, efficient and high-fidelity method to transfer protein-coding regions between Flexi® Vectors with various expression or peptide-tag options while maintaining insert orientation and reading frame. A lethal gene in the Flexi® Vector cloning region allows positive selection of clones containing insert. Antibiotic resistance genes simplify selection of the desired clone during subsequent transfers to other Flexi® Vectors.

Cell Notes 11, 13–16.

Related Products

Related Resources