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Clone and Express Protein-Coding Regions Using the Flexi® Vector Systems

Michael Slater, Jim Harnett, Natalie Betz, Jami English, Ethan Strauss, Becky Pferdehirt, and Elaine Schenborn
Promega Corporation
Publication Date: 2005

Abstract

The Flexi® Vector Systems provide a rapid, efficient and high-fidelity method to transfer protein-coding regions between Flexi® Vectors with various expression or peptide-tag options while maintaining insert orientation and reading frame. A lethal gene in the Flexi® Vector cloning region allows positive selection of clones containing insert. Antibiotic resistance genes simplify selection of the desired clone during subsequent transfers to other Flexi® Vectors.

Cell Notes 11, 13–16.

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