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New Sensitive Bioluminescent Caspase-Glo® 2 and 6 Assays: Using Inhibitors to Achieve...

New Sensitive Bioluminescent Caspase-Glo® 2 and 6 Assays: Using Inhibitors to Achieve Specificity in Cells
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Abstract

Our new, extremely sensitive, homogeneous luminescent caspase-2 and -6 apoptosis assays provide unique tools for defining the function of these elusive caspases. An understanding of cross-reactivity between caspases, their substrates, and the various peptide inhibitors is critical for interpreting results from apoptosis studies. Here we provide details on using the Caspase-Glo® 2 Assay  in cells.

Cell Notes 19, 8–13.

Martha O'Brien, Cassandra Broulette, Michael Scurria, Laurent Bernad and William Daily

Promega Corporation and Promega Biosciences

Publication Date: 2007

Download Article (316 KB)

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