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Human Cell Line Misidentification

Yvonne A. Reid, Ph.D.
Yvonne Reid joined ATCC in 1980 and during the mid-1980's her research focused on the use of DNA hypervariable probes for the interspecies identification of human cell lines. The evolution of this work has led to the implementation of STR analysis as a routine screening method of all human cell lines at ATCC and other cell banks.
Recently she co-chaired the ATCC Standard Development Organization (SDO) committee on the Development of a Consensus Standard for the Authentication of Human Cell Lines: Standardization of STR profiling.
Dr. Reid had more than 30 years of experience in cell biology, immunology and molecular biology. As Collection Scientist for the Cell Biology Program for over 10 years, she was responsible for the accessioning of new animal cell lines and hybridomas into the Cell Biology General Collection.
Dr. Reid has authored over 30 peer-reviewed publications and has served on more than a dozen scientific committees, including serving as an ad hoc member of the Comparative Medicine Review Committee, National Cell Culture for Resources (NIH); Chairperson, of ad hoc advisory member for the National Cell Culture Center, Minneapolis, MN, member of the 2005-2010 USP Fetal Bovine Serum as hoc Advisory Panel, and member of the 2010-2011 USP Cryopreservation ad hoc Advisory Panel.

 

Douglas R. Storts, Ph.D.
Dr Storts is responsible for strategy and implementation related to the development of reagent systems for nucleic acid amplification, expression analysis and genotyping (human forensic, paternity, clinical research and cell line authentication).
  • Presented by: Yvonne A. Reid, Ph.D. and Douglas R. Storts, Ph.D

  • (Dr. Reid, Manager Scientist, ATCC and Dr. Storts, Head Research - Nucleic Acid Technologies, Promega Corporation)

  • Original Webinar Date: Tuesday, August 13, 2013

This misidentification of human cell lines in research labs is a significant problem that may be costing the scientific community millions of dollars annually. This webinar will cover the new cell line authentication standards and provide an overview of the different options available for authenticating human cell lines.

Information

Human cell line misidentification is a common, costly problem in the research community. It typically occurs as a result of contamination in a laboratory that is manipulating several different cell lines, or due to mislabeling errors. Consequences include wasting a significant amount of time generating and publishing erroneous data. Many journals now recommend or require authentication of cell line identity before accepting manuscripts for publication. Short Tandem Repeat (STR) genotyping analysis is a proven technology for uniquely identifying human cell lines. The technology was recently recommended as the preferred technology for identification of human tissue culture cell lines by the ATCC Standards Development Organization (ASN-0002; Authentication of Human Cell Lines: Standardization of STR Profiling).

This webinar will cover (1) the cell line authentication and current guidelines, (2) principles if STR analysis, (3) a new collaborative cell line authentication service being done by ATCC and Promega, and (4) an overview of the GenePrint® 10 STR Profiling System specifically designed based on the ASN-0002 guidelines to enable individual researchers or core facilities to verify the identity of their human cell lines.

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