Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Reporter Gene Assays for the Investigation of Signal Transduction at Orexin Receptors

David Kosel and Lutz Weber
OntoChem GmbH
Publication Date: December 2012

Abstract

The neuropeptides orexin-A and -B are involved in the regulation of the circadian rhythm in mammals. Approximately 20-30% of people in western cultures suffer from insomnia. Therefore, the associated orexin-receptor system (OxR-system) is of strong pharmacological interest in drug discovery. The cell signaling receptors OxR1 und OxR2 belong to the family of G protein-coupled receptors. The luciferase reporter assay is an ideal tool to analyze the activity of OxR-ligands and affected cell signaling cascades.

How to Cite This Article

Scientific Style and Format, 7th edition, 2006

Kosel, D., Weber, L. Luciferase Reporter Assay for Deciphering Ligand Binding and Signaling of Orexin Receptors. [Internet] December 2012. [cited: year, month, date]. Available from: https://www.promega.com/resources/pubhub/applications-notes/reportergen-assays-zur-untersuchung-der-signaltransduktion-an-orexin-rezeptoren/

American Medical Association, Manual of Style, 10th edition, 2007

Kosel, D., Weber, L. Luciferase Reporter Assay for Deciphering Ligand Binding and Signaling of Orexin Receptors. Promega Corporation Web site. https://www.promega.com/resources/pubhub/applications-notes/reportergen-assays-zur-untersuchung-der-signaltransduktion-an-orexin-rezeptoren/ Updated December 2012. Accessed Month Day, Year.