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QuantiFluor™-P Fluorometer Method For DNA Quantitation Using Hoechst 33258

Abstract

The QuantiFluor™-P Handheld Fluorometer can be used for DNA quantitation along with Hoechst 33258, a bisbenzimide DNA intercalator that excites in the near UV (350nm) and emits in the blue region (450nm). Hoechst 33258 binds to the AT rich regions of double stranded DNA and exhibits enhanced fluorescence under high ionic strength conditions.