The Qubit® RNA Assay Kit can be used with the Quantus™ Fluorometer. The assay provides an accurate and selective method to quantify high-abundance RNA samples without quantitating DNA, protein or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents or protein, are well tolerated in the assay. The assay kit is designed to quantitate 5–100ng of RNA in a 200μl assay. This Application Note describes the protocol for using the Qubit® RNA Assay Kit with the Quantus™ Fluorometer.