Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Measuring Fluorescein using the Quantus™ Fluorometer

Publication Date: 10/13

Abstract

Fluorescein is a synthetic organic compound with wide-ranging biological application as a label for antibodies and other probes used in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. Because fluorescein is autofluorescent, no dye chemistries are necessary for quantitation on the Quantus™ Fluorometer. The only components required are a standard of known concentration and a blank. This Application Note describes a protocol for measuring fluorescein using the Quantus™ Fluorometer.

Part# AN228

Related Products