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Measuring Fluorescein using the Quantus™ Fluorometer

Publication Date: 10/13

Abstract

Fluorescein is a synthetic organic compound with wide-ranging biological application as a label for antibodies and other probes used in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. Because fluorescein is autofluorescent, no dye chemistries are necessary for quantitation on the Quantus™ Fluorometer. The only components required are a standard of known concentration and a blank. This Application Note describes a protocol for measuring fluorescein using the Quantus™ Fluorometer.

Part# AN228

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