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Internal Validation Guide for Y-STR Systems in Forensic Laboratories

Abstract

This guide lists a set of experiments for use or adaptation by laboratories performing an internal validation of a Y-STR amplification system. These studies are intended to demonstrate the sensitivity and reliability of the Y-STR system for development of Y-STR profiles. These experiments are sufficient to determine the following: optimal amount of male DNA input, the analytical threshold for your capillary electrophoresis (CE) instrument in combination with the amplification system, the lower limit of detection for reliable profile results, the effect of excessive amounts of female DNA on the efficiency of the amplification, and the impact of multiple males in a DNA profile.


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