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GloMax®-Multi Microplate Fluorometer Method to Quantify Living Colors® rAcGFP1

Abstract

Clontech Living Colors® recombinant green fluorescent protein from Aequorea coerulescens (rAcGFP1) is a new alternative to monomeric enhanced GFP (EGFP). The spectral properties of rAcGFP1 closely resemble those of EGFP; rAcGFP1, excitation maximum is 475nm and emission maximum is 505nm as compared to 484/510nm for EGFP. In addition, the rAcGFP1 protein has 94% homology to EGFP at the amino acid level and is very stable, allowing fluorescence examination over an extended period of time. rAcGFP1 can be used as a fluorescent marker for gene expression reporter assays in organisms varying from bacteria to higher plants and animals.