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GloMax®-Multi Jr Method for Quantitation of Living Colors® rAcGFP1 in Gene Expression

Abstract

Clontech Living Colors® green fluorescent protein from Aequorea coerulescens (AcGFP1) is a new alternative to monomeric enhanced GFP (EGFP). The spectral properties of AcGFP1 closely resemble those of EGFP, with AcGFP1 having an excitation maximum of 475nm and an emission maximum of 505nm (compared to 484/510nm of EGFP). In addition, the AcGFP1 protein has 94% homology to EGFP at the amino acid level and is very stable, allowing the examination of fluorescence over an extended period of time. AcGFP1 can be used as a fluorescent marker for gene expression in a variety of organisms from bacteria to higher plants and animals. Additionally, AcGFP1 can be used to visually monitor the real-time movement of your protein of interest.