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GloMax® 96 Microplate Luminometer Method for the Bright-Glo™ Assay

Abstract

The GloMax® 96 Microplate Luminometer in combination with the Bright-Glo™ Assay kit provides a convenient, rapid, and sensitive procedure for quantifying gene expression. Transcriptional regulation, coupled to the expression of a luciferase reporter gene, is regularly used to study a wide range of biological events in cultured cells. Luciferase is an ideal reporter because of the absence of endogenous luciferase activity in mammalian cells, and the functional enzyme is created immediately upon translation.