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GloMax® 96 Microplate Luminometer Method for Kinase-Glo® Luminescent Kinase Assay

Abstract

The GloMax® 96 Microplate Luminometer in combination with the Kinase-Glo® Luminescent Kinase Assay provides a convenient, rapid, and sensitive procedure for quantifying the amount of ATP remaining in solution after a kinase reaction. Kinases constitute a large class of enzymes that phosphorylate specific amino acids according to their original recognition motif. Consequently, kinases play a key role in cell signal transduction and represent exciting new targets for drug research. Scientific developments validate the potential utility of kinases, including serine/threonine, cAMP-dependent, casein, and MAP kinases as specific and important pharmaceutical leads.