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GloMax® 20/20 Luminometer Method for Renilla™-Glo Luciferase Assay System

Abstract

The GloMax® 20/20 Luminometer in combination with the Renilla-Glo™ Luciferase Assay System provides a convenient, rapid and sensitive procedure for quantifying gene expression. Transcriptional regulation, coupled to the expression of a luciferase reporter gene, is regularly used to study a wide range of biological events in cultured cells. Renilla luciferase (36kDa) is about half the size of firefly luciferase (61kDa), does not require ATP, and the functional enzyme is created immediately upon translation. The Renilla-Glo™ Luciferase Assay System specifically maximizes the sensitivity of the assay reagent while providing a luminescent signal half-life greater than 60 minutes.