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Rapid, Accurate and Flexible DNA Quantitation Using the QuantiFluor® dsDNA System on the MANTIS® Liquid Handler

Promega Corporation
Publication Date: June 2017

Abstract

Fluorescent dye-based quantitation systems are recommended for advanced downstream applications because of their sensitivity, target selectivity, cost, and ease of use. The QuantiFluor® dsDNA System can be used for quantitating dsDNA in single tubes as well as 96- and 384-well plates. Accuracy during repetitive pipetting of small volumes can be difficult and labor intensive with many opportunities for error and costly re-work. The MANTIS® is a novel bench-top liquid handler that can meet many requirements for high-throughput reagent dispensing at a fraction of the cost of larger systems. For nucleic acid quantitation, the MANTIS® accurately dispenses small volumes of the QuantiFluor® Dye working solution for reproducible quantitation results. In this application note, we demonstrate the compatibility of the MANTIS® liquid handler to quickly and accurately automate reagent handling of the QuantiFluor® dsDNA System for both 96- and 384-well plate formats.

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How to Cite This Article

Scientific Style and Format, 7th edition, 2006

Promega Corporation Rapid, Accurate and Flexible DNA Quantitation Using the QuantiFluor® dsDNA System on the MANTIS® Liquid Handler. [Internet] June 2017. [cited: year, month, date]. Available from: https://www.promega.com/resources/pubhub/applications-notes/dna-quantitation-using-the-quantifluor-dsdna-system-on-the-mantis-liquid-handler/

American Medical Association, Manual of Style, 10th edition, 2007

Promega Corporation Rapid, Accurate and Flexible DNA Quantitation Using the QuantiFluor® dsDNA System on the MANTIS® Liquid Handler. Promega Corporation Web site. https://www.promega.com/resources/pubhub/applications-notes/dna-quantitation-using-the-quantifluor-dsdna-system-on-the-mantis-liquid-handler/ Updated June 2017. Accessed Month Day, Year.

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