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Detection of Light-Induced cAMP Production in Living Cells

Tobias Eckhardt
Ruhr-Universität Bochum, Lehrstuhl für Allgemeine Zoologie und Neurobiologie
Publication Date: December 2012

Abstract

With optogenetic methods, signal transduction molecules can be activated using light and the affected signaling cascades can be analyzed in vivo. Signal transduction molecules, such as the photoactivatable adenylate cyclase bPAC of the sulfur bacterium Beggiatoa sp. produce the second messenger cyclical adenosine monophosphate (cAMP). The enzymatic formation of cAMP—and thereby the light-induced adenylate cyclase activity—can be detected with the GloSensor™ cAMP Assay using a luminescence signal.

How to Cite This Article

Scientific Style and Format, 7th edition, 2006

Eckhardt, T. Detection of Light-Induced cAMP Production in Living Cells. [Internet] December 2012. [cited: year, month, date]. Available from: https://www.promega.com/resources/pubhub/applications-notes/camp-produktion-in-lebenden-zellen/

American Medical Association, Manual of Style, 10th edition, 2007

Eckhardt, T. Detection of Light-Induced cAMP Production in Living Cells. Promega Corporation Web site. https://www.promega.com/resources/pubhub/applications-notes/camp-produktion-in-lebenden-zellen/ Updated December 2012. Accessed Month Day, Year.