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Monitoring Lipolysis Using the Glycerol-Glo Assay and GloMax Discover System

Abstract

The Glycerol-Glo™ Assay is well-suited for monitoring lipolysis via glycerol release in cultured adipocytes. The assay works by correlating glycerol molecules to light production. First, a coupled reaction catalyzes glycerol to generate NADH. Next, a reductase enzyme uses NADH to convert a proluciferin reductase substrate into luciferin. Finally, Ultra-Glo™ rLuciferase oxidizes luciferin to produce visible light. The amount of light produced can be measured using a luminometer such as the GloMax® Discover. In this way, the Glycerol-Glo™ Assay offers rapid and sensitive glycerol detection. It uses a simple add-and-read protocol and can be performed in 96- or 384-well plates, making it amenable to both low- and high-throughput adipocyte cell culture applications.

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