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Assaying Cell Health in 3D Cell Culture with the GloMax Discover System

Promega Corporation
Publication Date: 9/20


This Application Note describes the protocol to measure cell viability from cultured cells grown the GravityPLUS™ 3D Culture and Assay Platform and the GloMax® Discover System. The InSphero GravityPLUS™ 3D Culture and Assay Platform is a scaffold-free 3D culture system designed to grow microtissues from hanging drops.  The system contains plates to culture and assay microtissues. The Promega CellTiter-Glo® 3D and RealTime-Glo™ MT Cell Viability assays effectively assay for metabolically active cells through the luminescent measurement of cellular ATP and membrane potential, respectively.

Measuring the cell viability from 3D cultured cells can be easily performed using the GloMax® Discover detection system with integrated protocols for CellTiter-Glo® 3D and RealTime-Glo™ to provide an extended dynamic range and superior detection sensitivity. 

How to Cite This Article

Scientific Style and Format, 7th edition, 2006

Promega Corporation Assaying Cell Health in 3D Cell Culture with the GloMax® Discover System. [Internet] 9/20. [cited: year, month, date]. Available from: https://www.promega.com/resources/pubhub/applications-notes/an308-assaying-cell-health-in-3d-cell-culture-with-the-glomax-discover-system/

American Medical Association, Manual of Style, 10th edition, 2007

Promega Corporation Assaying Cell Health in 3D Cell Culture with the GloMax® Discover System. Promega Corporation Web site. https://www.promega.com/resources/pubhub/applications-notes/an308-assaying-cell-health-in-3d-cell-culture-with-the-glomax-discover-system/ Updated 9/20. Accessed Month Day, Year.

RealTime-Glo is a trademark of Promega Corporation. CellTiter-Glo and GloMax areregistered trademarks of Promega Corporation.
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