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Assaying Cell Health in 3D Cell Culture with the GloMax Discover System

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Abstract

This Application Note describes the protocol to measure cell viability from cultured cells grown the GravityPLUS™ 3D Culture and Assay Platform and the GloMax® Discover System. The InSphero GravityPLUS™ 3D Culture and Assay Platform is a scaffold-free 3D culture system designed to grow microtissues from hanging drops.  The system contains plates to culture and assay microtissues. The Promega CellTiter-Glo® 3D and RealTime-Glo™ MT Cell Viability assays effectively assay for metabolically active cells through the luminescent measurement of cellular ATP and membrane potential, respectively.

Measuring the cell viability from 3D cultured cells can be easily performed using the GloMax® Discover detection system with integrated protocols for CellTiter-Glo® 3D and RealTime-Glo™ to provide an extended dynamic range and superior detection sensitivity. 

Promega Corporation Publication Date: May 2017

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How to Cite This Article

Promega Corporation Assaying Cell Health in 3D Cell Culture with the GloMax® Discover System. [Internet] May 2017. [cited: year, month, date]. Available from: http://www.promega.com/resources/pubhub/applications-notes/an308-assaying-cell-health-in-3d-cell-culture-with-the-glomax-discover-system/

Promega Corporation Assaying Cell Health in 3D Cell Culture with the GloMax® Discover System. Promega Corporation Web site. http://www.promega.com/resources/pubhub/applications-notes/an308-assaying-cell-health-in-3d-cell-culture-with-the-glomax-discover-system/ Updated May 2017. Accessed Month Day, Year.

RealTime-Glo is a trademark of Promega Corporation. CellTiter-Glo and GloMax areregistered trademarks of Promega Corporation.
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