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Measuring ATP in Low Bioburden Water Samples Using the Water-Glo™ Kit

Publication Date: 2/20


Water used for manufacturing of consumer goods, beverages and pharmaceuticals must be tested for microbial contamination. Typically, such water has low bioburden. Traditional microbial compendial methods are based on culturing techniques that are labor-intensive and time-consuming, often requiring days of incubation. Therefore, production is seldom able to take proactive corrective action based on the results. In addition, culturing conditions may impact sensitivity, accuracy and reproducibility. Culture-independent rapid microbiological methods can overcome these challenges. One of these methods is based on detection of ATP, the cellular energy currency. Cellular ATP levels effectively correlate with viable biomass or metabolic activity. This protocol is designed to measure ATP from water samples that have low bioburden using the Water-Glo™ Kit (Cat.# AM1002).