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Direct Amplification of DNA from Storage Card Punches and Swabs Using the VersaPlex 27PY System

Publication Date: 1/20

Abstract

Direct amplification of known standard samples has become the preferred analysis method in laboratories. The ability of a laboratory to conduct direct amplification using the same kit utilized in casework can significantly streamline their workflow while stretching their budget. For this reason, the direct amplification application was examined for use with the VersaPlex™ 27PY System. Testing shows the VersaPlex™ 27PY System with the ProFlex® PCR System enables direct amplification with 12.5μl reactions from numerous sample types, including Whatman FTA® card punches, S&S 903 Paper, Bode Buccal DNA Collector™ devices, OmniSwabs and cotton swabs. This Application Note provides example protocols for direct amplification of DNA from storage card punches and swabs.

Part# AN354