Monitoring of microbial testing for sterile and non-sterile products, as well as sterility testing, requires microbial culturing with or without filtration. Traditional compendial microbial methods are based on culturing techniques which are labor-intensive and time-consuming. Such tests require several days of incubation for the microbial contamination to be detected. Therefore, management is seldom able to take proactive corrective action. In addition, culturing conditions may impact sensitivity, accuracy and reproducibility. To overcome these challenges, culture-independent, rapid microbiological methods are often considered. One of the methods relies on detection of ATP, the cellular energy currency. Cellular ATP levels can be effectively correlated to viable biomass or metabolic activity. This application note describes an overview of different workflows for rapid microbial analysis.