Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Using the NanoBiT® PPI System with GloMax® Discover to Detect Protein-Protein Interactions

Publication Date: 9/17


This Application Note presents an example protocol using the NanoBiT® PPI System and the GloMax® Discover multimode plate-reader to detect rapamycin-inducible FKBP:FRB protein interactions. Dose response of rapamycin was evaluated, and kinetics of the protein association were followed in real time.