Using the NanoBiT® PPI System with GloMax® Discover to Detect Protein-Protein Interactions

Publication Date: 9/17


This Application Note presents an example protocol using the NanoBiT® PPI System and the GloMax® Discover multimode plate-reader to detect rapamycin-inducible FKBP:FRB protein interactions. Dose response of rapamycin was evaluated, and kinetics of the protein association were followed in real time.