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Amplifications Using the PowerPlex® 16 System and 10X Primer Mix Stored TE–4 Buffer or in Water

Abstract

Promega is changing the solution used to prepare the 10X Primer Pair Mix in the PowerPlex® 16 System from water to TE–4 buffer. We show that the amplification results with the 10X Primer Pair Mix prepared in TE–4 buffer are comparable to those with the 10X Primer Pair Mix prepared in water, even when we varied reaction parameters (the amount of DNA template, volume of TE–4 buffer, the thermal cycler used,amount of AmpliTaq Gold® DNA polymerase used and primer concentrations). Because the performance with both primerpair formulations is comparable, users of the PowerPlex® 16 System should see no adverse effects on amplification results.

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