SIRT-Glo™ Assay System Technical Manual
Literature # TM336
The SIRT-Glo™ Assay System is a single-reagent-addition, homogeneous, luminescent assay that measures the relative activity of the NAD+-dependent histone deacetylase (HDAC) class III enzymes (sirtuins; SIRTs) from purified enzyme sources. This assay is broadly useful for class III enzymes, but sensitivity and performance will vary with catalytic efficiency of particular isoenzymes. The assay uses an acetylated, luminogenic peptide substrate that can be deacetylated by SIRT activities. Deacetylation of the peptide substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the peptide from aminoluciferin, which is quantified in a reaction using Ultra-Glo™ Recombinant Luciferase. The SIRT-mediated luminescent signal is persistent and proportional to deactetylase activity, allowing batch processing of multiwell plates.
Summary of Changes
The 2/18 version of this technical manual has been modified to remove any mention of discontinued products.
Printed in USA. Revised 2/18.