PowerPlex® Y23 System for Use on the Applied Biosystems® Genetic Analyzers Protocol
Literature # TMD035
The PowerPlex® Y23 System allows co-amplification and four-color fluorescent detection of 23 loci, including DYS576, DYS389I, DYS448, DYS389II, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643, DYS393, DYS458, DYS385a/b, DYS456 and Y-GATA-H4. The PowerPlex® Y23 System provides all materials necessary to amplify Y-STR regions of human genomic DNA, including a hot-start DNA polymerase, and is compatible with the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
Summary of Changes
The following changes were made to the 4/17 revision of this document:
1. Added support for half-volume direct-amplification reactions and use of the Veriti® 96-Well Thermal Cycler.
2. Added caution against storing PCR amplification mix prior to thermal cycling.
3. Revised document name to specify that this manual is for use on Applied Biosystems instruments.
4. Incorporated other general updates.
Printed in USA. Revised 4/17.