PowerPlex® ESI 17 Pro System for Use on the Applied Biosystems® Genetic Analyzers Technical Manual

Instructions for Use of Product(s)
DC7780

Literature # TMD036

The PowerPlex® ESI 17 Pro System was developed for human identification applications including forensic analysis, relationship testing and research use. The PowerPlex® ESI 17 Pro System allows co-amplification and four-color fluorescent detection of seventeen loci (sixteen STR loci and Amelogenin),including D22S1045, D2S1338, D19S433, D3S1358, Amelogenin, D2S441, D10S1248, D1S1656, D18S51, D16S539, D12S391, D21S11, vWA, TH01, SE33, FGA and D8S1179.
The PowerPlex® ESI 17 Pro System is an update to the PowerPlex® ESI 17 System (Cat.# DC6780, DC6781). All primer pairs, with the exception of the SE33 primer pairs, are identical in both systems. The internal lane standard, WEN Internal Lane Standard 500 ESS, is designed and optimized specifically for this system.

Summary of Changes
The following changes were made to the 9/21 revision of this document:
1. Removed discontinued Cat.# DC7781.
2. Added ProFlex® PCR System and removed GeneAmp® PCR System 2720.
3. Added instructions for using reaction tubes in amplification reactions (Section 4).
4. Updated Section 6.L.
5. Revised Section 7.
6. Incorporated other general updates.
7. Updated cover page.

Revised 9/21.