PowerPlex® ESI 16 System Technical Manual

Instructions for Use of Product(s)
DC6770, DC6771
Literature # TMD027

The PowerPlex® ESI 16 System allows co-amplification and four-color fluorescent detection of sixteen loci, including D18S51, D21S11, TH01, D3S1358, Amelogenin, D16S539, D2S1338, D1S1656, D10S1248, FGA, D8S1179, vWA, D22S1045, D19S433, D12S391 and D2S441. This system includes a proprietary 5X master mix that contains hot-start Taq DNA polymerase, buffer, salts, MgCl2 and dNTPs. The PowerPlex® ESI 16 System is compatible with the ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Summary of Changes: 5/16 Revision
The following changes were made to the 5/16 version of this document:
1. The kit component CC5 Internal Lane Standard 500 (CC5 ILS 500) was replaced with the WEN Internal Lane Standard 500 (WEN ILS 500). Instructions were updated throughout the document for use of this new internal lane standard.
2. The artifacts table in Section 6.L was updated.
3. Instructions for older versions of GeneScan® and Genotyper software were removed.
4. Other general updates were incorporated.

Printed in USA. Revised 5/16.