:

United States

United States

Choose language: English

PowerPlex® 18D System Technical Manual

Instructions for Use of Product(s)
DC1802, DC1808
Literature # TMD031

The PowerPlex® 18D System allows co-amplification and four-color fluorescent detection of eighteen loci, including D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Amelogenin, vWA, D8S1179, TPOX, FGA, D19S433 and D2S1338. The PowerPlex® 18D System provides all materials necessary to amplify STR regions of genomic DNA, including a hot-start, nonproofreading thermostable DNA polymerase. The system is optimized for analysis of common database samples, such as unwashed FTA® card punches (i.e., direct amplification). Additionally, thermal cycling time is reduced from previous PowerPlex® protocols. The PowerPlex® 18D System is compatible with the ABI PRISM® 3100 and 3100-Avant and Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Summary of Changes
The following changes were made to the 4/17 revision of this document:
1. Added support for half-volume direct-amplification reactions and use of the Veriti® 96-Well Thermal Cycler.
2. Added caution against storing PCR amplification mix prior to thermal cycling.
3. Revised document name to specify that this manual is for use on Applied Biosystems instruments.
4. Incorporated other general updates.

Printed in USA. Revised 4/17.

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom