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PowerPlex® 16 System Technical Manual

Instructions for Use of Product(s)
DC6530, DC6531

Literature # TMD012

The PowerPlex® 16 System allows co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin). The system contains the loci Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One primer specific for Penta E, D18S51, D21S11, TH01 and D3S1358 is labeled with fluorescein (FL); one primer specific for FGA, TPOX, D8S1179, vWA and Amelogenin is labeled with carboxy-tetramethylrhodamine (TMR); and one primer specific for Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 is labeled with 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxy-fluorescein (JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane.

Summary of Changes
The following change was made to the 4/22 version of this document:
1. Removed mention of ABI PRISM® Genetic Analyzers.
2. Added ProFlex® PCR System and removed Perkin Elmer model 480 and GeneAmp® PCR System 2400 and 9600 thermal cyclers (Section 5).
3. Updated Section 6.
4. Removed Section 9.C.
5. Revised Section 9.E.
6. Updated disclaimers.
7. Incorporated other general updates.
8. Placed document into a new template.

Revised 4/22.