Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

HDAC-Glo™ Class IIa Assay

Instructions for Use of Product(s)

Literature # TM407

The HDAC-Glo™ Class IIa Assay is a single-reagent-addition, homogeneous, luminescence assay that selectively measures the relative activity of histone deacetylase (HDAC) class IIa enzymes from cells, extracts or recombinant sources. The assay uses an isoenzyme-selective, acetylated, live-cell permeant, luminogenic peptide substrate that can be deacetylated by HDAC class IIa enzymes. Deacetylation of the peptide substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the peptide from aminoluciferin. The resulting luminescence is quantified in a reaction using Ultra-Glo™ Recombinant Luciferase.

Summary of Changes

The following changes were made to the 5/15 revision of this document:
1. Storage Conditions and Additional Storage Considerations were updated.

Revised 5/15.

Experienced User Protocols