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HDAC-Glo™ Class IIa Assay

Instructions for Use of Product(s)

Literature # TM407

The HDAC-Glo™ Class IIa Assay is a single-reagent-addition, homogeneous, luminescence assay that selectively measures the relative activity of histone deacetylase (HDAC) class IIa enzymes from cells, extracts or recombinant sources. The assay uses an isoenzyme-selective, acetylated, live-cell permeant, luminogenic peptide substrate that can be deacetylated by HDAC class IIa enzymes. Deacetylation of the peptide substrate is measured using a coupled enzymatic system in which a protease in the Developer Reagent cleaves the peptide from aminoluciferin. The resulting luminescence is quantified in a reaction using Ultra-Glo™ Recombinant Luciferase.

Summary of Changes

The following changes were made to the 5/15 revision of this document:
1. Storage Conditions and Additional Storage Considerations were updated.

Revised 5/15.

Experienced User Protocols