We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Differex™ System—For Use With the Differex™ Magnet Technical Manual

Instructions for Use of Product(s)
DC6800, DC6801
Literature # TM331

The Differex™ System is an easy and efficient method to separate epithelial and sperm DNA and reduces variability in results from one examiner to another, a problem common to other differential extraction methods. In addition, through the use of the DNA IQ™ Resin to cap the pellet, fewer sperm are lost during the process. The proteinase K digestion from the standard methodology is retained for the upfront processing of the solid support. The Differex™ Magnet has two different positions because the position and shape of the pellet formed after each centrifugation step differs slightly. The DNA IQ™ Resin migrates to the magnet and caps the sperm pellet. The two separate magnets allow the DNA IQ™ Resin to form a better cap over the pellets.
The total time to separate sperm from epithelial cells after adding the sample to the proteinase K-containing Digestion Solution is approximately 2 hours, which includes a 90-minute proteinase K digestion. DNA purification requires 30 minutes, so separation and purification can be accomplished in as little as 2.5 hours.

Summary of Changes
The following changes were made to the 9/16 revision of this document:
1. A note was added to Section 3 for clarity.
2. Part number V1221 was changed to V1225, and part number V4741 was changed to V4745.

Printed in USA. Revised 9/16.

Download video:

Closed format: MP4
Open format: OGG
Choose your country

Americas

Brazil
Canada
United States

Pacific Asia

Australia
India
Japan
Korea, Republic of
Singapore

Europe

Austria
Belgium
Denmark
Estonia
Finland
France
Germany
Iceland
Italy
Luxembourg
Netherlands
Norway
Poland
Spain
Sweden
Switzerland
United Kingdom